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| Registros recuperados: 233 | |
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| Bandeira, Juliana Magalhães; UFPel; Bianchi, Valmor João; UFPel; Rubin, Sílvia; UFPel; Peters, José Antonio; UFPel; Braga, Eugenia Jacira Bolacel; UFPel. |
| The Plectranthus genus comprises several species generally referred to as boldo, which are highly used in popular medicine due to their anti-dyspeptic, analgesic and digestion-stimulating properties. The amount of natural active principles can vary greatly in the related genotypes, which may lead to the inappropriate use of these plants. This work aimed to analyze the interspecific diversity among four species of the Plectranthus genus (P. grandis, P. barbatus, P. neochilus and P. amboinicus) and the intraspecific diversity of P. barbatus collected from different places in southern Brazil, by means of the RAPD technique. A higher genetic similarity was observed between the P. neochilus and P. amboinicus species (80%), followed by P. grandis and P. barbatus... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: RAPD; Boldo; Genetic diversity; Medicinal plants Botânica; Genética Vegetal; Genética Molecular e de Microrganismos; Ciências Biológicas. |
| Ano: 2009 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/880 |
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| Sebastianes,Fernanda Luiza de Souza; Maki,Cristina Sayuri; Andreote,Fernando Dini; Araújo,Welington Luiz; Pizzirani-Kleiner,Aline Aparecida. |
| The Pink Disease is caused by Erythricium salmonicolor, which attacks broad hosts, such as citrus, coffee, rubber, Eucalyptus spp. and Acacia spp., infecting mainly branches. This disease became a serious problem in Brazil, reducing the citrus production up to 10%. However the genetic diversity and compatibility of the fungus E. salmonicolor from Brazilian citrus plants is not yet evaluated. Therefore, the aims of this study were to evaluate: i) the genetic variability of E. salmonicolor in the São Paulo and Minas Gerais States by the RAPD technique, and ii) the vegetative compatibility between these isolates. After RAPD analysis, six distinct groups were observed without correlation between the isolation site or host species. In the vegetative... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Corticium salmonicolor; RAPD; Pink Disease; Hyphal fusion; Heterokariosis. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162007000200009 |
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| Ramella,Micheline Sandra; Kroth,Mariela Aparecida; Meurer,Samira; Nuñer,Alex Pires de Oliveira; Zaniboni Filho,Evoy; Arisi,Ana Carolina Maisonnave. |
| The genetic variability of four fish species (Pimelodus maculatus, Prochilodus lineatus, Salminus brasiliensis and Steindachneridion scripta) collected in the upper Uruguay River basin was analyzed using the RAPD technique. A total of 118 amplified fragments was obtained, 11 for P. maculatus, 29 for P. lineatus, 45 for S. brasiliensis and 33 for S. scripta. Amplified fragments with monomorphic profile were not found in the studied species, except for S. brasiliensis, which presented seven monomorphic bands for Saltinho population. All species showed high levels of genetic variability among individuals. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: RAPD; Pimelodus maculatus; Prochilodus lineatus; Salminus brasiliensis; Steindachneridion scripta. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500008 |
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| Oliveira,Vanice Dias; Rabbani,Allivia Rouse Carregosa; Silva,Ana Veruska Cruz da; Lédo,Ana da Silva. |
| This research had as objective to characterize genetically individuals of physic nut cultivated in experimental areas in Sergipe, Brazil by means of RAPD molecular markers. Leaves of 40 individuals were collected and DNA was isolated using CTAB 2% method. Were used 30 primers RAPD for DNA amplification, and this data was used to estimate the genetic similarity among the pairs of individuals, using Jaccard coefficient, and group them out for the UPGMA method. Also, the genetic structure and diversity of the populations were assessed using AMOVA. Of the 100 fragments generated, 29 of were polymorphic. A similarity average of 0.54 among the individuals was found and the amplitude similarities varied from 0.18 to 1.00. One of them (U5) was unit clusters and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Jatropha curcas L.; RAPD; Genetic diversity. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013000600006 |
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| Glienke-Blanco,Chirlei; Aguilar-Vildoso,Carlos Ivan; Vieira,Maria Lúcia Carneiro; Barroso,Paulo Augusto Vianna; Azevedo,João Lúcio. |
| During some phases of of their life-cycle endophytic fungi colonize plants asymptomatically being found most frequently inside the aerial part of plant tissues. After surface disinfection of apparently healthy leaves from three varieties of mandarin orange and one tangor, and after incubation on appropriate culture medium, 407 fungal isolates were obtained, giving a total infection frequency of 81%. No fungal growth was observed from disinfected seeds, indicating that fungi are probably not transmitted via seeds. Of the fungal isolates, 27% belonged to the genus Guignardia, with 12 isolates being identified as Guignardia citricarpa Kiely, which is described as a citrus pathogen. The isolates were variable in respect to the presence of sexual structures and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Citrus; Endophytes; Genetic variability; Guignardia citricarpa; Mandarin; RAPD. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200021 |
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| Panarari-Antunes,Renata de Souza; Prioli,Alberto José; Prioli,Sônia Maria Alves Pinto; Galdino,Alexsandro Sobreira; Julio Junior,Horácio Ferreira; Prioli,Laudenir Maria. |
| This study aimed to evaluate the genetic variability of B. orbignyanus in cultivated and natural populations of the Upper Paraná River, using molecular RAPD markers and mtDNA control region. Specimens were collected in the Paraná River and in the Piracema fish farm in Maringá, State of Paraná, Brazil. RAPD primers produced 82 loci with consistent expression. The population from the Paraná River showed 28 polymorphic loci, whereas the population from the fish farm presented only 12. Data revealed genetic differentiation between the two populations, although not very pronounced. These results were corroborated by the principal coordinate analysis and by neighbor-joining clustering. The alignment of the D-loop sequences of B. orbignyanus indicated the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Freshwater fish; Endangered; RAPD; MtDNA; Polymorphism. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400025 |
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| Torezan,José Marcelo Domingues; Souza,Rogério Fernandes de; Ruas,Paulo Maurício; Ruas,Claudete de Fátima; Camargo,Elvis Hassmann; Vanzela,André Luís Laforga. |
| RAPD was used to access the genetic variability in Aspisdosperma polyneuron, a long-lived, late-reproducing tropical tree, and highly important for the Atlantic Forest. RAPD profiles from adults (pre-fragmentation, >300 years old) and seedlings (post-fragmentation, <<50 years old) were analyzed. Results showed a decrease of genetic polymorphism of post-fragmentation cohorts in small fragments and higher genetic diversity within population. The genetic diversity distribution suggested the establishment of fragments as protected reserves, and the transference of seedlings among fragments for conservation of A. polyneuron. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aspidosperma; DNA markers; Forest fragmentation; Genetic variability; Peroba-rosa; RAPD. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000200002 |
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| Paiva,Samuel Rezende; Silvério,Vanessa Chaves; Egito,Andréa Alves; McManus,Concepta; Faria,Danielle Assis de; Mariante,Arthur da Silva; Castro,Sílvia Ribeiro; Albuquerque,Maria do Socorro Maués; Dergam,Jorge Abdala. |
| The objectives of this work were to investigate the genetic structure of the Brazilian hair sheep breeds and to determine the origin of the Santa Inês breed. Molecular similarity was determined using Randomly Amplified Polymorphic DNA - Polymerase Chain Reaction markers in 238 individuals from five naturalized sheep breeds: Santa Inês (48 animals), Rabo Largo (48), Somali (48), Morada Nova (48) and Bergamasca (46), collected in Goiás, Sergipe, Bahia, and Ceará States as well as in the Federal District. Fifty-four loci were selected from 19 primers, after a pilot test using 140 primers. Qualitative analyses indicate diagnostic markers for all breeds. All breeds were significantly different from each other. Interbreed differences were explained by 14.92% of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ovis aries; Conservation genetics; Domestic animal diversity; Molecular markers; RAPD. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2005000900008 |
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| Sahyun,Sandra Aparecida; Ruas,Eduardo Augusto; Ruas,Claudete de Fátima; Medri,Cristiano; Souza,José Roberto Pinto de; Johansson,Loana Aparecida Pereira da Silva; Miranda,Luíz Vicente; Ruas,Paulo Maurício. |
| Three populations of Maytenus aquifolium from Monte Alegre farm, Telemaco Borba county, Paraná, Brazil were analyzed by RAPD markers. A total of 13 primers were applied wich yielded 283 well amplified markers in all the studied populations (Mortandade, Vila Preta and Trinita), producing different values of gene diversity and polymorphic loci. The analysis of molecular variance (AMOVA) indicated that 21.77% of the genetic variation was among the population. Pairwise F ST analysis showed that the most divergent populations were closer geographically, demonstrating that other factors such as different soil types could explain this variation. Bayesian analysis for K number of clusters and the Principal Coordinate indicated that these three populations were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Conservation; Genetic diversity; Maytenus aquifolium; RAPD; Populations. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500005 |
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| WEIKERT-OLIVEIRA,RITA C. B.; RESENDE,M. APARECIDA DE; VALÉRIO,HENRIQUE M.; CALIGIORNE,RACHEL B.; PAIVA,EDILSON. |
| Twenty isolates of four fungal species, agents of "Helminthosporium" diseases in cereals, were collected from different regions: nine Bipolarisoryzae isolated from rice (Oryza sativa), seven B.sorokiniana from wheat (Triticum aestivum), two B. maydis, and two Exserohilumturcicum from maize (Zea mays). The strains were compared by PCR-RFLP and RAPD analysis. Size polymorphism among the isolates in the ITS region comprising the 5.8 S rDNA indicated genetic differences among the isolates, while a UPGMA phenogram constructed after the digestion of this region with restriction enzymes showed inter- and intra-specific polymorphism. The RAPD profiles indicated an expressive level of polymorphism among different species, compared with a low level of polymorphism... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bipolaris sorokiniana; Bipolaris oryzae; Bipolaris maydis; Exserohilum turcicum; PCR-RFLP; RAPD. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582002000600015 |
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| Ranjan,Sanjeev Kumar; Mallick,Chandana Basu; Saha,Dipnarayan; Vidyarthi,Ambarish S; Ramani,Ranganathan. |
| The lac insects (Homoptera: Tachardiidae), belonging to the genus Kerria, are commercially exploited for the production of lac. Kerria lacca is the most commonly used species in India. RAPD markers were used for assessing genetic variation in forty-eight lines of Kerria, especially among geographic races, infrasubspecific forms, cultivated lines, inbred lines, etc., of K. lacca. In the 48 lines studied, the 26 RAPD primers generated 173 loci, showing 97.7% polymorphism. By using neighbor-joining, the dendrogram generated from the similarity matrix resolved the lines into basically two clusters and outgroups. The major cluster, comprising 32 lines, included mainly cultivated lines of the rangeeni form, geographic races and inbred lines of K. lacca. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: DNA fingerprinting; Genetic variation; Kerria; Lac insects; RAPD; Tachardiidae. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000300023 |
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| Almeida,Álvaro M. R.; Abdelnoor,Ricardo V.; Arias,Carlos A. Arrabal; Carvalho,Valdemar P.; Jacoud Filho,David S.; Marin,Silvana R. R.; Benato,Luís C.; Pinto,Mauro C.; Carvalho,Cláudio G. P.. |
| Macrophomina phaseolina has been considered one of the most prevalent soybean (Glycine max) pathogens in Brazil. No genetic resistance has been determined in soybean and very little is known about the genetic diversity of this pathogen in tropical and sub-tropical regions. Fifty-five isolates from soybean roots were collected in different regions and analyzed through RAPD for genetic diversity. The UPGMA cluster analysis for 74 loci scored permitted identification of three divergent groups with an average similarity of 99%, 92% and 88%, respectively. The three groups corresponded to 5.45%, 59.95% and 34.6%, respectively of all isolates used. A single plant had three different haplotypes, while 10.9% of the analyzed plants had two different haplotypes. In... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: RAPD; ITS; PCR-RFLP; Soybean charcoal rot. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582003000300009 |
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| Santos,Ronaldo Pereira; Ângelo,Paula Cristina da Silva; Sampaio,Paulo de Tarso Barbosa; Quisen,Regina Caetano; Leite,Ângela Maria Conte; Oliveira,Cristiane Lopes de. |
| Rosewood (Aniba rosaeodora Ducke, Lauraceae) is an Amazonian evergreen tree and a source of the purest linalool, the main component of its essential oil, which is very valuable in the international perfumery market. After decades of over-exploitation it is currently considered as threatened. We evaluated the genetic diversity and its distribution in four populations in Central Amazonia. Thirty-five reliable RAPD markers were generated, of which 32 were polymorphic (91.4%). Variation was higher within the populations (76.5%; p < 0.0001) and geographic distribution contributed to population differentiation (23.4%; p < 0.0001). The Amazon River had a small influence on gene flow (3.3%; p < 0.0001), but we identified evidence of gene flow across the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: RAPD; Linalool; Gene flow; Amazon Rainforest. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672008000300010 |
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| Stringari,Danyelle; Glienke,Chirlei; Christo,Daniel de; Maccheroni Jr.,Walter; Azevedo,João Lucio de. |
| RAPD markers were used to investigate the distribution of genetic variability among a group of Guignardia citricarpa, G. mangiferae, and Phyllosticta spinarum isolates obtained from several hosts in Brazil, Argentina, Mexico, Costa Rica, Thailand, Japan, United States and South Africa. Pathogenic isolates G. citricarpa Kiely (anamorph form P. citricarpa McAlp Van Der Aa) are the etiological agent of the Citrus Black Spot (CBS), a disease that affects several citric plants and causes substantial injuries to the appearance of their fruits, thus preventing their export. Several previous studies have demonstrated the existence of an endophytic species with high morphological similarity to the causal agent of CBS that could remain latent in the same hosts.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Guignardia; SCARS; Citrus Black Spot; Citrus; Diagnosis; RAPD. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500002 |
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| Registros recuperados: 233 | |
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